Ambizione @snsf_ch project leader Green organisms - Looking at tiny frozen mitochondria with big microscopes @Biozentrum - Engel Lab . 🇨🇵-🇩🇪-🇨🇭
Bâle, Suisse
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Super excited to share our latest work on the native in-cell organization of the mitochondrial respiratory chain 🥳
Using cryo-electron tomography🔬, we show how the respiratory complexes (and other complexes) are organized inside native mitochondria!
biorxiv.org/content/10.1101/…
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Florent Waltz retweeted
New OA Article: "Chloroplast-encoded small subunit extensions reshape the Chlamydomonas chlororibosome" rdcu.be/fwoSj
In situ cryo-electron tomography reveals a unique ‘arm’ domain on the small subunit of Chlamydomonas chlororibosomes.
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Long in the making, but happy to present the Chlamydomonas chlororibosome!
Cryo-ET🔬reveals a new domain on the small subunit, made by multiple extensions in conserved ribosomal proteins. Chlororibosomes might be more diverse than expected!
bioRxiv 📖: shorturl.at/q44tG
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Florent Waltz retweeted
How plant mitochondria make their own proteins 🌱🔬
A new study published in @NatureComms reveals the structure of the cauliflower mitoribosome and a unique RsgA mechanism that blocks premature translation.
▶️nature.com/articles/s41467-0…
#PlantBiology #CryoEM #Ribosome
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I had a wonderful time in Japan 🗼!
Hugethanks to @ken_wakaba for inviting me to Sangyo University in Kyoto. Thanks to @algalccm (and Ken-ichi) for organizing the Beyond the Green 🍀 session at the Japanese Botanical Society meeting!
Great science and discussions!
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Florent Waltz retweeted
Check out our new preprint on the discovery of a molecular switch in NAC that mediates nascent chain sorting on the ribosome and prevents mitochondrial protein mistargeting by SRP. A great collaboration with the Shan Lab @Caltech and the Qi Lab @UVA: biorxiv.org/content/10.1101/…
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You want to start tomography? Solve structures inside cells? Reach Nyquist😳?
We have a website for you! tomoguide.github.io
You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomo averaging, using different software!
Hope it will be useful !
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Florent Waltz retweeted
In-cell architecture of the mitochondrial respiratory chain. @Science Check the #cryoEM #structure of this #membrane #protein in the UniTmp database: pdbtm.unitmp.org/entry/9fy5
doi.org/10.1126/science.ads8…
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Florent Waltz retweeted
Molecular machines enable efficient energy production in mitochondria. Using cryo-electron tomography @bengeliscious and @FlorentWaltz have gained insight into the architecture of mitochondria at unprecedented resolution. @ScienceMagazine @snsf_ch
ow.ly/irOA50Vm1xK
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The final version of our work on the in-cell architecture of the mitochondrial respiratory chain is now online in @ScienceMagazine 🎉
You can find the full story here science.org/doi/10.1126/scie…
@luminous_lab did a wonderful job on the cover and animation👩🎨
In mitochondria, respiratory supercomplexes (blue) pump protons across crista membranes to create an electrochemical gradient, fueling adenosine triphosphate (ATP) synthase (pink) to regenerate ATP—the energy currency of life.
Now, cryo–electron tomography captures these supercomplexes in their native organization, illuminating how the coordinated action of these molecular machines drives life’s fundamental energy conversion.
Learn more this week in Science: scim.ag/3FQ6bEh
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Florent Waltz retweeted
技術は「何に使うか」が大事。開発した葉緑体ゲノム編集技術で、光合成/CO2固定の律速酵素RubiscoのL-subunitの改良、植物生育促進に挑戦&成功しました！光合成/Rubicso/生化学/構造科学の先生方（矢守/松村/深山）との超絶共同研究の成果です！biorxiv.org/content/10.1101/…
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Florent Waltz retweeted
Part of this data was also used in preprint from @FlorentWaltz on the native in-cell organization of the mitochondrial respiratory chain with some impressive sub-nm resolution respirasome structures
Super excited to share our latest work on the native in-cell organization of the mitochondrial respiratory chain 🥳
Using cryo-electron tomography🔬, we show how the respiratory complexes (and other complexes) are organized inside native mitochondria!
biorxiv.org/content/10.1101/…
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Florent Waltz retweeted
Excited to share our new preprint and 1800+ tomograms with the community! 🎉 This is our first step towards building a visual proteomics dataset for Chlamydomonas reinhardtii. I hope the community will be able to make good use of this data!🔬#CryoET
biorxiv.org/content/10.1101/…
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Florent Waltz retweeted
It's a great pleasure to share our latest collaborative work detailing the composition and structure of the highly rRNA-fragmented mitoribosome from Apicomplexa, such as Plasmodium falciparum and Toxoplasma gondii, now out in @NatureComms
rdcu.be/d3R8C
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Florent Waltz retweeted
We are excited to share our latest work on the cooperation between RNA polymerase and the ribosome in bacteria: science.org/doi/10.1126/scie…
The work was supported by @IGBMC, @Inserm, @CNRS, @unistra, @ERC_research, @IMC_Bio, and @instructhub. 1/7
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How are mRNAs recruited to the ribosome small subunit in bacteria? Super cool story highlighting the role of bS1. Long standing basic questions answered there 🔬
science.org/doi/10.1126/scie…
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Florent Waltz retweeted
Our lab at @cnrs_ibmp is delighted to announce the @NatureComms publication from a PhD student and postdoc-driven collaboration with @jlaw_lab and @FlorentWaltz revealing how CLSY proteins dock with Pol IV to produce siRNAs and silence transposons: nature.com/articles/s41467-0…
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Cool stuff there ⬇️
Our new preprint is out: we solved the structures of HIV-1 matrix (MA) from intact virions by SPA. The structure led us to a surprising discovery resolving a long mystery in HIV-1 biology – peptide SP2 regulates the maturation of the HIV-1 matrix. 🧵1/x
tinyurl.com/c7szhtfa
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Finally got some time to properly test #RELION5 complete pipeline, starting from raw frames to STA. 22 tilt-series (from EMPIAR-11830), picking done with pytom, 14600 final particles, FSC 4.1-ish on the LSU (Nyquist bin1 3.8). Ofc it's a ribosome 😅 but now I can try with smth ..
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